human adenocarcinoma ovary cell line skov 3  (ATCC)

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Punicalagin Inhibits the Growth and Proliferation of Ovarian Epithelial Adenocarcinoma Cells Via Apoptosis and Autophagic Cell Death

doi: 10.1002/jbt.70908

Figure Lengend Snippet: Punicalagin dose‐dependently decreases the growth of ovarian cancer cells. The ovarian cancer cell lines, OVCAR‐3 and SKOV‐3, were treated with PCG (from 6.25 to 200 µM) for 24, 48, and 72 h. Cell proliferation was detected by the WST assay. PCG caused a decrease in the growth of (A–C) OVCAR 3 cells and (D–F) SKOV‐3 cells with varying IC 50 s. IC 50 dose concentrations are presented in (G–I) for OVCAR‐3 cells and (J–L) SKOV‐3 cells. Following the statistical analysis, the data in the graphs have been derived from at least three repeated experiments and shown as the mean ± SD. * p ≤ 0.05 compared to control.

Article Snippet: Ovarian cancer cell lines SKOV‐3 and OVCAR‐3 were obtained from ATCC (Manassas, USA).

Techniques: WST Assay, Derivative Assay, Control

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Punicalagin Inhibits the Growth and Proliferation of Ovarian Epithelial Adenocarcinoma Cells Via Apoptosis and Autophagic Cell Death

doi: 10.1002/jbt.70908

Figure Lengend Snippet: Punicalagin suppressed the colony formation ability of ovarian cancer cells. The ovarian cancer cell lines, OVCAR‐3 and SKOV‐3, were treated with PCG (from 6.25 to 200 µM) for 48 h and then incubated for 14 days. PCG causes a decrease in the colony formation ability of the (A) OVCAR‐3 and (B) SKOV‐3 cells. The inhibitory effect was more prominent in the OVCAR‐3 as compared to SKOV‐3 cells.

Article Snippet: Ovarian cancer cell lines SKOV‐3 and OVCAR‐3 were obtained from ATCC (Manassas, USA).

Techniques: Incubation

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Punicalagin Inhibits the Growth and Proliferation of Ovarian Epithelial Adenocarcinoma Cells Via Apoptosis and Autophagic Cell Death

doi: 10.1002/jbt.70908

Figure Lengend Snippet: Punicalagin decreased the migration ability of ovarian cancer cells. The wound healing assay showed a decrease in the ability of ovarian cancer cells to migrate after PCG treatment. PCG caused such an effect in 48 h in (A) OVCAR‐3 cells and for 24 and 48 h in (B) SKOV‐3 cells. The highest cell migration inhibitory effects were obtained in the SKOV‐3 cells as compared to OVCAR‐3 cells. Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.

Article Snippet: Ovarian cancer cell lines SKOV‐3 and OVCAR‐3 were obtained from ATCC (Manassas, USA).

Techniques: Migration, Wound Healing Assay, Control

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Punicalagin Inhibits the Growth and Proliferation of Ovarian Epithelial Adenocarcinoma Cells Via Apoptosis and Autophagic Cell Death

doi: 10.1002/jbt.70908

Figure Lengend Snippet: Punicalagin suppressed the transwell migration ability of ovarian cancer cells. To assess the transwell migration ability of PCG on the ovarian cancer cells, the OVCAR‐3 and SKOV‐3 were subjected to 48 h to PCG at various doses (50, 100, and 200 µM). PCG treatment resulted in the decreased transwell migration ability of the (A) OCAR‐3 and (B) SKOV‐3 cells. (C) Western blot assay revealed a decrease in the N‐cadherin, while an increase in E‐cadherin was observed in SKOV‐3 cancer cells. Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.

Article Snippet: Ovarian cancer cell lines SKOV‐3 and OVCAR‐3 were obtained from ATCC (Manassas, USA).

Techniques: Migration, Western Blot, Control

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Punicalagin Inhibits the Growth and Proliferation of Ovarian Epithelial Adenocarcinoma Cells Via Apoptosis and Autophagic Cell Death

doi: 10.1002/jbt.70908

Figure Lengend Snippet: Punicalagin disrupted the mitochondrial membrane potential in ovarian cancer cells. Representative images for the analysis of MMP through the JC‐10 assay in (A) OVCAR‐3 cells and (B) SKOV‐3 cells. All cells were seeded at a density of 4 × 10 3 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.

Article Snippet: Ovarian cancer cell lines SKOV‐3 and OVCAR‐3 were obtained from ATCC (Manassas, USA).

Techniques: Membrane, Cell Culture, Control

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Punicalagin Inhibits the Growth and Proliferation of Ovarian Epithelial Adenocarcinoma Cells Via Apoptosis and Autophagic Cell Death

doi: 10.1002/jbt.70908

Figure Lengend Snippet: Punicalagin caused an increase in ROS production in ovarian cancer cells. For the quantification of the ROS, the ovarian cancer cells were subjected to PCG for 48 h before employing the MitoSOX assay. There was an increase in the ROS generation at higher doses for (A) OVCAR‐3 and (B) SKOV‐3 cells. Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.

Article Snippet: Ovarian cancer cell lines SKOV‐3 and OVCAR‐3 were obtained from ATCC (Manassas, USA).

Techniques: Mitosox Assay, Control

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Punicalagin Inhibits the Growth and Proliferation of Ovarian Epithelial Adenocarcinoma Cells Via Apoptosis and Autophagic Cell Death

doi: 10.1002/jbt.70908

Figure Lengend Snippet: Punicalagin caused apoptosis in ovarian cancer cells. Representative images for the analysis of Annexin V/PI assay in (A) OVCAR‐3 and (B) SKOV‐3 cells. All cells were seeded at a density of 8 × 10 4 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). (C) Representative image of the BAX expression in the SKOV‐3 cells. Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.

Article Snippet: Ovarian cancer cell lines SKOV‐3 and OVCAR‐3 were obtained from ATCC (Manassas, USA).

Techniques: Cell Culture, Expressing, Control

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Punicalagin Inhibits the Growth and Proliferation of Ovarian Epithelial Adenocarcinoma Cells Via Apoptosis and Autophagic Cell Death

doi: 10.1002/jbt.70908

Figure Lengend Snippet: Punicalagin causes autophagy in ovarian cancer cells. Representative images for the analysis of autophagy through Acridine Orange staining in (A) OVCAR‐3 and (B) SKOV‐3 cells. All cells were seeded at a density of 4 × 10 3 per well in cell culture dishes and treated with (0.1% DMSO) and PCG (50, 100, and 200 µM). Punicalagin caused autophagy in the OVCAR‐3 cell line. (C) Representative image of the BAX expression in the OVCAR‐3 cells. Data were obtained after statistical analysis using at least three repeated experiments, presented in graphs as the mean ± SD. * p ≤ 0.05 compared to control.

Article Snippet: Ovarian cancer cell lines SKOV‐3 and OVCAR‐3 were obtained from ATCC (Manassas, USA).

Techniques: Staining, Cell Culture, Expressing, Control